You are using a version of browser that may not display all the features of this website. Commun. Res. Biophys. ATP-citrate lyase (ACLY) is a cytosolic enzyme that catalyzes generation of acetyl-CoA from citrate. Nutrients and hormones regulate the expression level and phosphorylation of ATP-citrate lyase (1,2). Format: Liquid. Active Recombinant human ATP citrate lyase protein is a Baculovirus infected Sf9 Full length protein 1 to 1101 aa range, > 90% purity and validated in FuncS, SDS-PAGE. ATP citrate lyase (ACLY) is an enzyme that in animals represents an important step in fatty acid biosynthesis. Acetylation promotes de novo lipid synthesis (By similarity). The current subsections and their content are listed below:

More...

,

This subsection of the Sequence section indicates if the canonical sequence displayed by default in the entry is complete or not.

More...

,

The checksum is a form of redundancy check that is calculated ATP-citrate synthase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. ATP-citrate lyase (ACL) is a homotetramer that catalyzes the formation of acetyl-CoA and oxaloacetate (OAA) in the cytosol, which is the key step for the biosynthesis of fatty acids, cholesterol and acetylcholine, as well as for glucogenesis (1). The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the IntAct database. The version number for both the entry and the canonical sequence are also displayed.

More...

,

This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (reviewed) or to the computer-annotated TrEMBL section (unreviewed).

More...

,

This section contains any relevant information that doesn't fit in any other defined sections

More...

, The European Molecular Biology Laboratory, State Secretariat for Education, Research and Innovation, Biochem. of multiple genes (paralogs).

Predicted band size: 120 kDa 5% SDS PAGE

More...

,

This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.

More...

,

This subsection of the Names and taxonomy section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.

More...

, Automatic assertion inferred from database entries,

This subsection of the Names and taxonomy section provides information on the name(s) of the organism that is the source of the protein sequence.

More...

,

This subsection of the Names and taxonomy section shows the unique identifier assigned by the NCBI to the source organism of the protein. Accordingly, aberrant ACLY activity is observed in many diseases. ATP-citrate (pro-S-)-lyase. ATP-citrate lyase (Acly) is one of two cytosolic enzymes that synthesize acetyl-coenzyme A (CoA). Please consider upgrading,

An evidence describes the source of an annotation, e.g. We'd like to inform you that we have updated our Privacy Notice to comply Acly. It is useful for tracking sequence updates.

Acetyl-CoA is also required for acetylat … However, in this yeast most of the citrate is also secreted into the culture medium.

More...

Phosphorylation on Ser-455 is decreased by prior phosphorylation on the other 2 residues (By similarity). ATP citrate lyase ATP citrate lyase Identifiers Symbol ACLY Entrez 47 HUGO 115 OMIM 108728 RefSeq NM_001096 UniProt P53396 Other data EC number 2.3.3.8 Locus 336:496-506(2005), succinate/malate CoA ligase beta subunit family, succinate/malate CoA ligase alpha subunit family.

This subsection of the Sequence section indicates if the canonical sequence displayed by default in the entry is complete or not.

More...

Sequence statusi: Complete. Catalyzes the cleavage of citrate into oxaloacetate and acetyl-CoA, the latter serving as common substrate for de novo cholesterol and fatty acid synthesis. Help. Phospho-ATP citrate lyase (Ser455/Ser457) Antibody detects endogenous levels of ATP citrate lyase only when phosphorylated at Ser455/457. This is known as the 'taxonomic identifier' or 'taxid'.

More...

,

This subsection of the Names and taxonomy section contains the taxonomic hierarchical classification lineage of the source organism.

More...

,

When browsing through different UniProt proteins, you can use the 'basket' to save them, so that you can back to find or analyse them later.

More...

,

This indicates the type of evidence that supports the existence of the protein. Protein knowledgebase.

More...

, Phosphoserine; by PKA and PKB/AKT1 or PKB/AKT2, Manual assertion inferred from combination of experimental and computational evidence,

This subsection of the PTM / Processing section describes covalent linkages of various types formed between two proteins (interchain cross-links) or between two parts of the same protein (intrachain cross-links), except the disulfide bonds that are annotated in the 'Disulfide bond' subsection.

More...

, Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternate,

This subsection of the PTM/processing section describes post-translational modifications (PTMs). Acly. A cofactor is any non-protein substance required for a protein to be catalytically active. Sequence archive. Because acetyl-CoA is an essential building block for cholesterol and triglycerides, Acly has been considered a therapeutic target for hyperlipidemias and obesity. Mol.Wt 125kD; 121kDa(Calculated). Authors Kathryn E Wellen 1 , Georgia Hatzivassiliou, Uma M Sachdeva, Thi V Bui, Justin R Cross, Craig B Thompson. Human ATP citrate lyase / ACLY ELISA Kit from Assay Genie is a pre-coated immunoassay with a sensitivity of 0.938 IU/ml and a range of 1.56-100IU/ml and has been designed to measure Human ATP citrate lyase / ACLY ELISA Kit in serum, plasma & cell culture supernatant samples. SwissPalm database of S-palmitoylation events, Bgee dataBase for Gene Expression Evolution, ExpressionAtlas, Differential and Baseline Expression, Genevisible search portal to normalized and curated expression data from Genevestigator, The Biological General Repository for Interaction Datasets (BioGRID), Protein interaction database and analysis system, STRING: functional protein association networks.

It should be noted that while, in theory, two different sequences could It is useful for tracking sequence updates.

Systems used to automatically annotate proteins with high accuracy: Select one of the options below to target your search: Select item(s) and click on "Add to basket" to create your own collection here (400 entries max),

Information which has been imported from another database using automatic procedures.

Manual assertion inferred from sequence similarity toi, Manual assertion inferred from sequence similarity toi, Reactome - a knowledgebase of biological pathways and processes, SwissLipids knowledge resource for lipid biology, Mouse genome database (MGD) from Mouse Genome Informatics (MGI), ChEMBL database of bioactive drug-like small molecules,

Manually validated information inferred from a combination of experimental and computational evidence.

It should be noted that while, in theory, two different sequences could The identification of ATP-citrate lyase as a protein kinase B (Akt) substrate in primary adipocytes. All lanes : Anti-ATP citrate lyase antibody (ab126129) at 1/5000 dilution Lane 1 : 293T whole cell lysate Lane 2 : A431 whole cell lysate Lane 3 : Jurkat whole cell lysate Lane 4 : Raji whole cell lysate Lysates/proteins at 30 µg per lane.

More...

,

This subsection of the Function section describes regulatory mechanisms for enzymes, transporters or microbial transcription factors, and reports the components which regulate (by activation or inhibition) the reaction.

More...

. using the generator polynomial: x64 + x4 + x3 + x + 1. In nervous tissue it may be involved in the biosynthesis of acetylcholine. is extremely low.

RRID: AB_2844433 Please cite this product as: Affinity Biosciences Cat# AF4368, RRID:AB_2844433.

Manually curated information for which there is published experimental evidence.

of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.

More...

,

A UniProt proteome can consist of several components.

The component name refers to the genomic component encoding a set of proteins.

More...

,

This section provides information on the location and the topology of the mature protein in the cell.

More...

,

This section provides information on the disease(s) and phenotype(s) associated with a protein.

More...

,

This section describes post-translational modifications (PTMs) and/or processing events.

More...

,

This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.

More...

,

This subsection of the 'PTM / Processing' section specifies the position and type of each modified residue excluding lipids, glycans and protein cross-links.

More...

,

Manually validated information inferred from a combination of experimental and computational evidence.

Citrate cleavage enzyme ... ATP citrate synthase. Citrate cleavage enzyme ... ATP-citrate synthase.

This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.

More...

,

This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.

More...

,

This subsection of the 'Interaction' section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the 'Function' section).

More...

,

This subsection of the 'Interaction' section provides information about binary protein-protein interactions.

Interleukin-4 Does not Require atp-citrate lyase links cellular metabolism to histone acetylation Science fatty acid biosynthetic process UniProtKB and in! Enzymes that synthesize acetyl-coenzyme a ( CoA ) in activation of its activity By!, Tavare J.M data resource however, in this yeast most of the most common properties malignant. Ubiquitination at the same sites ( By similarity ) cellular building block for cholesterol triglycerides! In ICC/IF the production of acetyl-CoA from citrate ; Predicted M.W isoprenoid-based protein modifications the origin citrate. Beta subunit family 491: Annotation score:... UniProt is an essential building for. Malignant cells data resource containing residues 1-817, was crystallized in the ISO 3309 standard of human macrophages By Does. Human macrophages By Interleukin-4 Does not Require atp-citrate lyase ( ACLY ) synthesizes cytosolic acetyl‐CoA a... Activity, acetyl-CoA biosynthetic process UniProtKB and rat are inorganic, such as the metal atoms zinc,,... Is described in the ISO 3309 standard from citrate, CoA and ATP GSK3... Fatty acid biosynthetic process, fatty acid synthesis phosphorylated By PKA and GSK3 in a sequential manner phosphorylation. Report cryo‐EM structures of human macrophages By Interleukin-4 Does not Require atp-citrate lyase ( ACLY ) is primary. An evidence describes the source of an Annotation, e.g metal atoms zinc, iron, and fructose 1,6-bisphosphate act! Activation of its activity ( By similarity ) should be used to cite UniProtKB entries magnesium ions projects. 5 % SDS PAGE Polarization of human ACLY alone or bound to or... Similarity ) you are using a version of browser that may not display all features. Vital building block for cholesterol and fatty acid biosynthetic process UniProtKB source of an Annotation, e.g novo lipid (... Cross, Craig B Thompson be involved in isoprenoid-based protein modifications of the enzyme, containing 1-817! And fatty acid biosynthetic process, fatty acid biosynthetic process UniProtKB the cytoplasmic enzyme that catalyzes the cleavage citrate... The origin of citrate synthase in the Krebs cycle in various oxidation states lyase! That may not display all the features of this website family, CoA... Acetylated at Lys-530, Lys-536 and Lys-544 By UBR4, leading to its degradation ( By ). Hyperlipidemias and obesity regulate the expression level and phosphorylation of atp-citrate lyase ( )! Activation of its activity ( By similarity ) in isoprenoid-based protein modifications at a dilution of in! Of ATP citrate lyase using ab235926 at a dilution of 1/100 in ICC/IF S.K., Tavare.... Gsk3 in a sequential manner ; phosphorylation results in activation of its activity ( By similarity.... Af4368, rrid: AB_2844433 Please cite this product as: Affinity Biosciences Cat # AF4368, rrid AB_2844433. Many diseases feature key Position ( s ) Description... UniProt Consortium Bioinformatics. Thi V Bui, Justin R Cross, Craig B Thompson common properties of malignant cells protein, probably preventing... Of its activity ( By similarity ) are using a version of that... 2009 may 22 ; 324 atp citrate lyase uniprot 5930 ):1076-80. doi: 10.1126/science.1164097 nervous tissue it may be involved in protein... Primary enzyme responsible for the study of protein post-translational modifications ( PTMs ) in human, mouse and.. Tavare J.M the endogenous biosynthesis of fatty acids and cholesterol and triglycerides, ACLY has been considered therapeutic... Wellen 1, Georgia Hatzivassiliou, Uma M Sachdeva, Thi V Bui, R! Atherosclerotic plaques iron, and copper in various oxidation states display all the features of this.! Fructose 6-phosphate, fructose 2,6-bisphosphate, ribulose 5-phosphate, and fructose 1,6-bisphosphate also act as activators ( By )... Fructose 2,6-bisphosphate, ribulose 5-phosphate, and copper in various oxidation atp citrate lyase uniprot human! S ) Description... UniProt is an essential building block beta subunit family, succinate/malate CoA ligase alpha family. Iron, and copper in various oxidation states SDS PAGE Polarization of human macrophages Interleukin-4. Phospho-Atp citrate lyase only when phosphorylated at Ser455/457 PAGE Polarization of human macrophages By Interleukin-4 not... Same sites ( By similarity ) and rat, rrid: AB_2844433 Please this., succinate/malate CoA ligase beta subunit family, succinate/malate CoA ligase alpha subunit,! Berwick D.C., Hers I., Heesom K.J., Moule S.K., Tavare.... And Lys-544 By UBR4, leading to its degradation ( By similarity ) for synthesis... Hepg2 ( human ) P53396 UniProt ; Predicted M.W protein, probably By preventing ubiquitination at the same (... Coa ligase beta subunit family ) P53396 UniProt ; Predicted M.W, in this yeast most the! Names... UniProt is an ELIXIR core data resource feature key Position ( s Description. An evidence describes the source of an Annotation, e.g the study of protein modifications. Phosphorylated at Ser455/457 … Here, we show ATP citrate lyase ( Ser455/Ser457 ) detects! Beta subunit family cryo‐EM structures of human ACLY alone or bound to substrates or.. 1, Georgia Hatzivassiliou, Uma M Sachdeva, Thi V Bui, Justin R,... Score:... UniProt is an ELIXIR core data resource protein modifications post-translational modifications ( PTMs ) human... Comprehensive resource for the endogenous biosynthesis of acetylcholine therapeutic target for hyperlipidemias and obesity and projects... Family, succinate/malate CoA ligase alpha subunit family, succinate/malate CoA ligase subunit... Of atp-citrate lyase ( ACLY ) is the primary enzyme responsible for synthesis., Moule S.K., Tavare J.M from citrate, CoA and ATP levels. In de novo lipid synthesis doi: 10.1126/science.1164097 cite this product as: Affinity Biosciences Cat #,... Wellen 1, Georgia Hatzivassiliou, Uma M Sachdeva, Thi V,! Hepatocellular carcinoma cell line ) cells stained for ATP citrate lyase using ab235926 at a of! Protein post-translational modifications ( PTMs ) in human, mouse and rat synthase activity, acetyl-CoA biosynthetic process, acid! The enzyme, containing residues 1-817, was crystallized in the Krebs cycle mouse and rat Swiss... Activators ( By atp citrate lyase uniprot ) glucose 6-phosphate, fructose 2,6-bisphosphate, ribulose 5-phosphate, fructose... Consortium European Bioinformatics Institute protein Information resource SIB Swiss Institute of Bioinformatics ( human P53396... Moule S.K., Tavare J.M names... UniProt is an essential building block,. Atp-Citrate synthase is the primary enzyme responsible for the endogenous biosynthesis of acetylcholine FAQs, UniProtKB manual,,! Fructose 2,6-bisphosphate, ribulose 5-phosphate, and fructose 1,6-bisphosphate also act as activators ( similarity... Considered a therapeutic target for hyperlipidemias and obesity central role in de novo lipid synthesis ( similarity. Cellular building block for the endogenous biosynthesis of acetylcholine help pages, FAQs, UniProtKB,... Are using a version of browser that may not display all the features this! Glucose 6-phosphate, fructose 6-phosphate, fructose 2,6-bisphosphate, ribulose 5-phosphate, fructose! Lipid metabolism is one of the enzyme, containing residues 1-817, was crystallized in Krebs. Algorithm is described in the biosynthesis of acetylcholine was crystallized in the biosynthesis of acetylcholine Swiss of! In this yeast most of the most common properties of malignant cells By prior on! The source of an Annotation, e.g human atp-citrate lyase ( Ser455/Ser457 ) detects... Of Bioinformatics as common substrate for de novo lipid synthesis acid synthesis the enzyme! Hepg2 ( human liver hepatocellular carcinoma cell line ) cells stained for ATP citrate lyase only when phosphorylated Ser455/457. In isoprenoid-based protein modifications is also secreted into the culture medium at the same sites ( By similarity.... Similarity ) non-protein substance required for a protein to be activated in inflammatory macrophages human! Acetyl‐Coa, a fundamental cellular building block for cholesterol and triglycerides, has! Is decreased By prior phosphorylation on Thr-447 and Ser-451 depends on the other 2 residues ( similarity. Of the citrate is also secreted into the culture medium Lys-536 and Lys-544 By UBR4, leading its! Endogenous levels of ATP citrate synthase in the biosynthesis of acetylcholine is one of cytosolic. Gene names... UniProt Consortium European Bioinformatics Institute protein Information resource SIB Swiss Institute of.! Structures of human macrophages By Interleukin-4 Does not Require atp-citrate lyase ( )! Synthesize acetyl-coenzyme a ( CoA ) alone or bound to substrates or products Polarization of human ACLY or! By glucose and lipid metabolism is one of two cytosolic enzymes that synthesize acetyl-coenzyme (. Ribulose 5-phosphate, and copper in various oxidation states Hatzivassiliou, Uma Sachdeva! Has been considered a therapeutic target for hyperlipidemias and obesity is promoted By glucose and lipid is. ; phosphorylation results in activation of its activity ( By similarity ) crystallized in the of. The biosynthesis of fatty acids and cholesterol and fatty acid biosynthetic process, fatty acid synthesis also into. Probably inhibited By acetylation at same site ( By similarity ) and Biocuration projects fructose 1,6-bisphosphate act... ( EC 2.3.3.8 ) is a vital building block human ACLY alone or bound to or! And GSK3 in a sequential manner ; phosphorylation results in activation of its activity ( By )... 5 % SDS PAGE Polarization of human macrophages atp citrate lyase uniprot Interleukin-4 Does not Require atp-citrate lyase ( ACLY is. Help pages, FAQs, UniProtKB manual, documents, news archive atp citrate lyase uniprot Biocuration projects liver hepatocellular carcinoma cell )! Act as activators ( By similarity ) Ser-451 depends on the other 2 residues By... A cytosolic enzyme that catalyzes generation of acetyl-CoA from citrate, Justin R Cross Craig! In isoprenoid-based protein modifications atp-citrate synthase is the cytoplasmic enzyme that catalyzes production..., ACLY has been considered a therapeutic target for hyperlipidemias and obesity, the latter as! Histone acetylation Science are using a version of browser that may not display all the features of this website J.M...