confidence in the strain origin, investigators used the C57BL/6 strain as the basis for large-scale biological resources such as genomic libraries. Takenaka et al. C57BL/10 Inbr (J) 158. They are more sensitive to noise and odours and are more likely to bite than the more docile laboratory strains such as BALB/c. Female 58 mated with the same male gave rise to strain C58. The chimeric mice are crossed to C57BL/6 to recover heterozygous deficient mice of C57BL/6 x 129 background. threne into the pancreas tissue of 90 C57BL/6 and 60 BALB/c mice, 13 developed ductal adenocarcinomas. We herein hypothesized that immune rejection of the transplanted iPSC-derived cardiomyocytes (iPSC-CMs) of allogeneic origin may be quantitated using 18F-DPA-714-PET imaging study. Over the last eight decades, The Jackson Laboratory has raised and distributed countless C57BL/6J (000664) mice. All procedures were approved by our institutional animal studies committee. Fluorescence‐activated cell sorting (FACS) confirmed that BD is nontoxic, and an exceptional cell labelling dye that opens up precision deep‐organ in vivo imaging of inflammation in mice routinely used for biomedical research. Allografts were analyzed for cell infiltration, CD8+T cell activation, fibrogenesis, and CAR using immunohistochemistry, Western blot, … All mice included in the study were females C57BL/6 and were obtained from the internal divisional stock (derived from C57BL/6j mice obtained from both The Jackson Laboratory and NIA/Charles River as well as from C57BL/6JRj mice from Janvier). Tumor volumes were assessed biweekly. Six mice per group were anesthetized with pentobarbital sodium and intranasally inoculat-ed with 105.5 TCID 50 virus or virus dilution buffer. Two of these tumors, both of C57BL/6 origin (Panc 02 and 03), were established in serial s.c. transplant. 2,18,22 The lesions are due to severe pruritus … Application for CD45.1 and CD45.2 cells. Female 58 mated with the same male gave rise to strain C58. U.S. The Df1 progeny of this mating (hereafter referred to as F 1 hybrid) are different than those of matings between mice of the mixed genetic background reported previously , in which the haploid region of chromosome 16 could be of C57BL/6 or 129SvEv origin. C57BL/6 Inbr (J) 150. Wild-type C57BL/6 mice normally carry the Ptprc b (CD45.2) allele while the other (congenic) strain carries the Ptprc a (CD45.1) allele on the same C57BL/6 genetic background. Two of these tumors, both of C57BL/6 origin (Panc 02 and 03), were established in serial s.c. transplant. The mice were euthanized by CO 2 hypoxia and weighed. Owing to genetic drift these colonies differ widely from one another (and, it goes without saying, from the original mice isolated at the Bussey Institute). C57BL/6, often referred to as "C57 black 6", "C57" or "black 6", is a common inbred strain of laboratory mouse. Groups of 15 female C57BL/6 mice were obtained at 4, 8, 12, 16, 20, and 24 weeks of age (Taconic, Germantown, NY, U.S.A.). Male C57BL/6 mice were selected as an appropriate model to study endogenous, ... Qiwen Li, Yunshu Wu, Ning Kang, Marrow Adipose Tissue: Its Origin, Function, and Regulation in Bone Remodeling and Regeneration, Stem Cells International, 10.1155/2018/7098456, 2018, (1-11), (2018). Femora and tibiae were isolated and cleaned of soft tissue. Female 58 mated with the same male gave rise to strain C58. The apoE(−/−) donor mice (C57BL/6 × 129) were backcrossed nine generations in C57BL/6 background. Strains 6 and 10 separated about 1937. In a previous study, it was observed that when C57BL/6 mice purchased from different vendors were infected with Plasmodium yoelii, a causative agent of murine malaria, they exhibited both differential immune responses and significantly different parasite burdens: these patterns … United States - 208A - Frederick, MD ; United States - 231 - Dublin, VA ; United States - 237 - Livermore, CA ; Download growth curve . The HF diet increased body weight (BW) and BW gain, as well as fasting glucose, total cholesterol, and hepatic lipid levels, compared to the LF diet-fed group. This substrain is now probably the most widely used of all inbred strains. There is some evidence that the lymphoblastic lymphomas ( Fig. The origin of enhanced performance is identified with the molecular structure and the distinct localisation of the dye within cells that enable remarkable changes … Mice that have been barbered have large bald patches on their bodies, commonly around the head, snout, and shoulders, although barbering may appear anywhere on the body. The black 6 mouse, above, was developed around 1920 by Clarence Cook Little (1881-1971), below. Molecules 2014, 19 12816 2. During the late 1980s, various groups led by Martin Evans, Mario Capecchi and Oliver Smithies created the first knockout mice. BALB/c or C57BL/6 hearts were heterotopically transplanted into WT, Clec9a−/−, or Batf3−/−recipient C57BL/6 mice. C57BL/6 male mice were randomly assigned to three diets for 7 weeks: low fat (LF, 16% kcal from fat), HF (HF, 60% kcal from fat), or HF with 5% ginger powder in diet (HF + G). Here, we demonstrated using for the first time electron paramagnetic resonance spectroscopy that black-spotted C57BL/6 spleens contain eumelanin. The lungs were then heated to 68uC for 24 hours and Despite recent advances in genome engineering technologies, traditional transgenic mice generated on a mixed genetic background of C57BL/6 and 129/Sv mice remain widely used in age-related hearing loss (AHL) research, since C57BL/6 mice exhibit early onset and progression of AHL due to a mutation in cadherin 23-encoding gene (Cdh23753G>A). [1], The dark coat make the mouse strain convenient for creating transgenic mice: it is crossed with a light-furred 129 mouse, and the desirable crosses can be easily identified by their mixed coat colors.[1]. The substrain "6" was the most popular of the surviving substrains. Despite its obvious limitations in mimicking everything that goes on within the human body, the laboratory mouse has proven to be an invaluable model of the human condition. The inbred strain of C57BL mice was created in 1921 by C. C. Little at the Bussey Institute for Research in Applied Biology. Two of these tumors, both of C57BL/6 origin (Pane 02 and 03), were estab lished in serial s.c. transplant. [5], The C57BL/6 mouse was the second-ever mammalian species to have its entire genome published. [1] Its overwhelming popularity is due largely to inertia: it has been widely used and widely studied, and therefore it is used even more. All the anomalies detected in C57BL/6 mice, including the origin of the septal artery from the left CA, have been previously described in two mutant mouse models: the iv/iv mouse (Icardo & Colvee 2001) and the Cx43 knockout mouse (Li et al. (c) Haplotype analysis identified contiguous regions of non-shared polymorphic alleles between KaLwRij and C57BL/6 mice (red bars) in 419 genes. In 1946, to the Jackson Laboratory, Bar Harbor. [8], Bussey Institute for Research in Applied Biology, "The Trouble With Black-6: A tiny alcoholic takes over the lab", "Aurora's Guide to Mouse Colony Management", "Targeted disruption of the MHC class II Aa gene in C57BL/6 mice", "LIFTOFF: FALCON 9 AND DRAGON BEGIN CRS-6 MISSION TO RESUPPLY THE SPACE STATION", Laboratory animal suppliers in the United Kingdom, Center for Alternatives to Animal Testing, Physicians Committee for Responsible Medicine, https://en.wikipedia.org/w/index.php?title=C57BL/6&oldid=982972055, Articles with unsourced statements from June 2014, Creative Commons Attribution-ShareAlike License, This page was last edited on 11 October 2020, at 13:52. B, mean tumor size is shown for each group. Strain of Origin: C57BL/6: Chromosome: 8: General Note: Expression of this human class I molecule in transgenic mice does not result in expansion of the number of cytotoxic T lymphocyte (CTL) precursors specific for other human class I Ag, HLA-B27 or HLA-A2.2. Female GF C57BL/6 mice were conventionalized at 4 weeks of age. The C57BL/6 mouse (1921) Despite its obvious limitations in mimicking everything that goes on within the human body, the laboratory mouse has proven to be an invaluable model of the human condition. The C57BL/6 inbred strain was developed starting in 1921 by Clarence Little at the Jackson Labs. probability-assisted coronary ligation in C57BL/6 mice.Am J Physiol Heart Circ Physiol 286: H1201–H1207, 2004; 10.1152/ajpheart. Strain Origin Developed by C.C. Following implant of cotton thread-carrying 3-methyl-cholanthrene into the pancreas tissue of 90 C57BL/6 and 60 BALB/c mice, 13 developed ductal adenocarcinomas. [1], Group-housed B6 female mice display barbering behavior, in which the dominant mouse in a cage selectively removes hair from its subordinate cage mates. H&E stained sections show the presence of tumor cells in the ovary (a-d) and peritoneum (i-l). Maint. This dark-furred rodent is sensitive to pain and cold, likes to drink alcohol when offered it, and tends to suffer from atherosclerosis and age-related hearing loss. C57BL/6J mice were purchased from the ORIENT BIO Animal Center (Seongnam-si, Korea) and housed in a dark/light cycle at Jeju National University. For the longevity study, 40 mice were randomly selected at 70 weeks of age. We have studied the origin and course of the main coronary arteries of two C57BL/6 mouse strains. Strains 6 and 10 separated prior to 1937. Responsible scientists, including those at accredited repositories, are careful to point out this fact and take pains to distinguish sublines such as C57BL/6J (the established subline at The Jackson Laboratory) from C57BL/6N, etc. C57BL/6 (CD45.2 +) mice, congenic C57BL/6 CD45.1 + mice, MCSF-R/GFP C57BL/6 mice (macrophage fas-induced apoptosis; Burnett et al., 2004) and BXH2 C57BL/6J C3H/HeJ mice that carry a spontaneous point mutation (R294C) in the transcriptional regulator Icsbp1/IRF8 (Icsbp1 C294; Turcotte et al., 2005) were purchased from The Jackson Laboratory. 4. The model was created by microinjecting the human CETP gene into (C57BL/6J x SJL) F2 zygotes. Colonization of female GF C57BL/6 mice does not impact osteoclast outgrowth from bone marrow cells. C57BL/6-derived alleles confer increased production of ANA, anti-ssDNA and anti-chromatin antibodies. By far the most popular laboratory rodent, the C57BL/6 mouse accounts for half to five-sixths of all rodents shipped to research laboratories from American suppliers. Both hair and whiskers may be removed. A homozygous colony is maintained for the production of hemizygotes through mating to C57BL/6NTac (N11)." by J,N, Ola. In contrast, the plasma cholesterol levels achieved in C57BL/6 mice were much lower, and atherosclerotic lesions were found almost exclusively in the aortic origin. They are the most widely used and best-selling mouse strain, due to the availability of congenic strains, easy breeding, and robustness.[1]. But has anyone wondered why this particular mouse, first bred in 1921 by Jackson Laboratory founder C.C. As Daniel Engber, writing in Slate, has noted: “From its start, the Black-6 was meant to be as reliable as a rubber stamp, but Little had grander aims for his identical, inbred mice. Mutant Mice Albino C57BL/6 Origin The albino C57BL/6 model was obtained from Allan Bradley at Baylor College of Medicine by the National Cancer Institute (NCI) in 2000. Little in 1921 from a mating of Miss Abbie Lathrop’s stock that also gave rise to strains C57BR and C57L. Unfortunately, Little’s reputation became somewhat tarnished for his support of eugenics and his denial of the links between tobacco and cancer. CD45.1 and CD45.2 cells are widely used in transplant and adoptive transfer studies to track the donor and host cells through the different alleles. Maint. … Wild-type C57BL/6 mice normally carry the Ptprc b (CD45.2) allele while the other (congenic) strain carries the Ptprc a (CD45.1) allele on the same C57BL/6 genetic background. To The Jackson Laboratory from Hall in 1948. a) C57BL/6 is a standard inbred strain, commonly used in transgenic breeding b) mate 6 – 8 week-old mice for best reproductive performance i. replace males when they are 1 year old ii. Control C57BL/6 recipient were transplanted with C57BL/6 donor hearts (C57BL/6 → WT C57BL/6, syngeneic transplants). iPSC-CM cell-sheets of C57BL/6 mice origin were transplanted on the surface of the left ventricle (LV) of C57BL/6 mice as a syngeneic cell-transplant model (syngeneic Tx group), or Balb/c mice as an … +136. (129S6/SvEvTac x C57BL/6)F1 hybrid … Backcrossing to C57BL/6NTac recommenced at Taconic in 2004. The same cross gave rise to strains C57L and C57BR. C57BL/6 mice have a dark brown, nearly black coat. Two of these tumors, both of C57BL/6 origin (Panc 02 and 03), were established in serial s.c. transplant. T: +44 (0)20 3019 5901, Studying immunology at undergraduate level, Studying immunology at postgraduate level, EFIS Young Immunologists Task Force (yEFIS). Strain of Origin: C57BL/6J: Chromosome: 12: General Note: C57BL/6 carries the responsive Ahr b allele; DBA/2 carries nonresponsive Ahr d. Heterozygotes (Ahr b /Ahr d) are responsive (J:5282). Quantities: Volume pricing is automatically applied when a minimum quantity per strain for a shipment is reached C57BL/6 mice. All mice included in the study were females C57BL/6 and were obtained from the internal divisional stock (derived from C57BL/6j mice obtained from both The Jackson Laboratory and NIA/Charles River as well as from C57BL/6JRj mice from Janvier). (b) Number of C57BL/6 and KaLwRij mice with positive M-spike by SPEP. [1][4] Unlike most mouse strains, it drinks alcoholic beverages voluntarily. Backcrossing to C57BL/6NTac recommenced at Taconic in 2004. The same cross gave rise to strains C57L and C57BR. 2006). The presence of melanin in spleens of black C57BL/6 mice has been known for long. 18,26,41 Ulcerations generally present on the dorsal scapulae, although torso, shoulder, and facial lesions are seen also, and may be single or multifocal in distribution. origin: Strain of Specimen: C57BL/6: Variant description: Allele Type: QTL : Notes : Mapping and Phenotype information for this QTL, its variants and associated markers J:93297. The identification of these compounds was performed by comparison of their mass spectra with those of the Wiley 7 and NIST 08, spectral libraries, as well as by comparison of … 18,26,41 Ulcerations generally present on the dorsal scapulae, although torso, shoulder, and facial lesions are seen also, and may be single or multifocal in distribution. Pane 02 was treated with 37 different anticancer drugs representing all of … Recipient mice were depleted of CD4 + T cells with anti‐CD4 antibody, i.p. Tested negative for bacteria, fungi, and mycoplasma. [6], In 2013 C57BL/6 mice were flown into space aboard Bion-M No.1. Cell lines derived from C57BL/6 mice are tumorigenic in SCID mice. Results and Discussion 2.1. Until recently, transmissible spongiform encephalopathy (TSE) in cattle was believed to be caused by a single strain of infectious agent identified at the beginning of a foodborne epidemic of bovine spongiform encephalopathy (BSE). B6C3F1, C57BL/6, B6;129 and FVB mice do not develop thymic lymphomas in young mice while 5–10% of aging CD-1 mice develop lymphomas in young mice (Son and Gopinath, 2004). He wanted one kind of animal to stand in for all the others in the lab: a mouse that served as a tiny cow, sure, but also a rabbit, a dog and a monkey. Harlan Laboratories acquired the model from the NCI in 2008. Credit: COURTESY OF THE JACKSON LABORATORY ARCHIVES As a boy, Clarence Cook Little kept mice … This was the strain of mouse whose genome was first sequenced in 2002, eighty-one years after it was created by American geneticist Clarence Cook Little in 1921 working in the neo-gothic splendour of the Bussey Institute at Harvard University. Black, a. Registered charity - 1043255 in England and Wales / SC047367 in Scotland, and registered in England and Wales as company 3005933, E: BSI@immunology.org [3], C57BL/6 has many unusual characteristics that make it useful for some work and inappropriate for other: It is unusually sensitive to pain and to cold, and analgesic medications are less effective in it. Molecular Note: The transgene contains the full length HLA-A2.1 gene. We herein hypothesized that immune rejection of the transplanted iPSC-derived cardiomyocytes (iPSC-CMs) of allogeneic origin may be quantitated using 18F-DPA-714-PET imaging study. Harlan became Envigo in 2015. (1 mg/mouse) on days ‐1, 0, and 7 and mice were analyzed on day 20 ( n = 5–10 per group). Origin: Little 1921 from the mating of female 57 with male 52 from Miss Abbie Lathrop's stock. ABSTRACT Following implant of cotton thread-carrying 3-methyl-cholan- threne into the pancreas tissue of 90 C57BL/6 and 60 BALB/c mice, 13 developed ductal adenocarcinomas. C57BL/6 mice are widely used for in vivo studies of immune function and metabolism in mammals. Finally, mice were sacrificed (six DOCA-treated 129/Sv, eight DOCA-treated C57BL/6, five DOCA-treated F1, six DOCA-treated F2, six 129/Sv controls, eight C57BL/6 controls, five F1 controls and six F2 controls). 00862.2003.—The ability to create experimental myocardial infarc-tions of reproducible size and location is tantamount to progress in multiple facets of ischemic heart disease research. Reasons to use C57BL/6 mice. The ROSA β-geo 26 mice is an engineered strain that shows ubiquitous expression of the Escherichia coli β-galactosidase from a lacZ gene promoter trap , providing a marker for identification of cells of donor origin by flow cytometry . Keywords: ginger; obesity; adipocyte remodeling 1. Individual MOVCAR cell lines isolated from C57BL/6 mice (MOVCAR 12, 5612, 5447 and 5438) were tested for tumorigenicity in SCID mice by i.p. Availability of resources such as this in turn drove the expansion of the use of the B6 strain for other endeavors. Strain Origin Developed by C.C. Strains 6 … But even within these sublines, the potential for drift exists in colonies maintained by individual laboratories who do not have a systematic practice of reestablishing breeders from a centralized, vetted stock. Black Subline 6 (BL6) became the most popular for scientific research and they were subsequently bred in their millions for scientific research around the world. [1,2]. Perhaps no strain of mouse exemplifies this best than a highly inbred mouse strain known as C57BL/6, sometimes abbreviated to “black 6” or B6. Credit: COURTESY OF THE JACKSON LABORATORY ARCHIVES" />The black 6 mouse, above, was developed around 1920 by Clarence Cook Little (1881-1971), below. (a) Phylogenetic tree demonstrating genetic distances of 12 inbred strains of mice. Mating brother and sister mouse for generation after generation and selecting the most vigorous pups for further in-breeding, Little eventually produced many distinct sublines that were almost as closely related to each other as genetic clones. Introduction Obesity, which has reached epidemic proportions world-wide, can cause a number of complications (elevated blood pressure, dyslipidemia, and insulin resistance etc.) Linkage analysis was performed on 141 female animals from a (129S6/SvEvTac x C57BL/6)F2 intercross to identify QTLs associated with systemic lupus erythematosus phenotypes. Unusual anatomical coronary arterial patterns were found, including: solitary ostium in aorta, accessory ostium, high take-off, aortic intramural course, slit-like ostium, sinus-like ostium and origin of a septal artery from the left coronary artery. Excessive fat accumulation is characterized by obesity and occurs when energy consump-tion is higher than energy expenditure [3]. Little is known about the origin of cDCs in nonlymphoid tissues. Harlan became Envigo in 2015. At that time, a “black subline” (C57BL) and a “brown subline” (C57BR) were established and bred independently. We previously reported that cattle were affected by a prion disorder that differed from bovine spongiform encephalopathy (BSE) by showing distinct molecular features of disease-associated protease-resistant prion protein (PrPres). It is the most widely used "genetic background" for genetically modified mice for use as models of human disease. There now exist colonies of mice derived from the original C57BL/6 colony that have been bred in isolation from one another for many hundreds of generations. C57BL/6 Mouse Mesenchymal Stem Cells derived from bone marrow of C57BL/6 mice that have a strong capacity for expansion. His inbred legions would take the place of humans, too, in testing of new drugs and treatment for cancer, tuberculosis and every other disease of man or mammal.”. To obtain the Df1 deletion on a congenic C57BL/6 c−/c− background (hereafter referred to as C57BL/6), we back-crossed Df1/+ mice for nine generations with C57BL/6 c−/c− inbred mice. Later work identified a second (J:8895) and later a third (J:22144) allele conferring response. However, several studies suggests that monocytes ... and monocytes to the development of nonlymphoid tissue cDCs, we injected pre-cDCs, monocytes, CDPs, and MDPs from C57BL/6 mice into untreated congenic recipient mice. Multipotential differentiation ability along osteogenic, chondrogenic and adipogenic lineages. Ulcerative dermatitis (UD) is a common syndrome of unknown etiology that results in profound morbidity in C57BL/6 mice and strains on a C57BL/6 background. The mice were then backcrossed one generation to C57BL/6 prior to intercrossing for homozygosity. The inbred strain of C57BL mice was created in 1921 by C. C. Little at the Bussey Institute for Research in Applied Biology. A, C57BL/6 mice ( n = 9–11/group) were inoculated with B16 on day 0 and treated with 250 mg/kg of cyclophosphamide on day 7 followed by 0.5 mg of αTRP1 or isotype control (C44) biweekly for 3 weeks. But his highly inbred, little black mouse lives on in labs around the world where scientists study the complexities of the mammalian immune system. Taconic's B6 Provides Superior Performance One of the most used inbred mouse models, the C57BL/6 is used in nearly every research application, and it's commonly used as the genetic background for transgenic mouse models. Linkage analysis was performed while controlling for Sle16 on Chromosome 1 and the LOD score increased from LOD=5.4 to LOD=6.4. Taconic received stock in April 1996 at the second backcross from the founder line. The mice were then backcrossed one generation to C57BL/6 prior to intercrossing for homozygosity. Ulcerative dermatitis (UD) is a common syndrome of unknown etiology that results in profound morbidity in C57BL/6 mice and strains on a C57BL/6 background. Strains 6 and 10 separated prior to 1937. At 5 days post-infection (DPI), the wet weight of the lungs from the mice was measured. Sle18 was also detected in the (129S6/SvEvTac x C57BL/6)F2-Apcs tm1Mpe intercross populationwith LOD=1.8. Bone marrow cells were flushed from the femurs of 8-week-old GF and CONV-D mice and stimulated for differentiation with RANKL and M-CSF. Inbr (J) ? Although its origin and biological functions are still obscure, the relation of splenic melanin to the hair follicle and skin pigmentation was suggested. CD4 + CD25 + cells were freshly isolated from spleens of C57BL/6 mice engrafted with 2 × 10 5 B16 cells 17 days before. [1], In 1993 the first C57BL/6 gene targeted knockout mouse was published by Frank Koentgen. Substrain 6 and 10 differ at the H9, Igh2 and Lv loci. Perhaps no strain of mouse exemplifies this best than a highly inbred mouse strain known as C57BL/6, sometimes abbreviated to “black 6” or B6. We show that intracerebral injection of such isolates into C57BL/6 mice produces a disease with preservation of PrP res molecular features distinct from BSE. Fluorescence‐activated cell sorting (FACS) confirmed that BD is nontoxic, and an exceptional cell labelling dye that opens up precision deep‐organ in vivo imaging of inflammation in mice routinely used for biomedical research. C57BL/6JOlaHsd; C57BL/6JRccHsd; C57BL/6NHsd; Locations . We show that intracerebral injection of such isolates into C57BL/6 mice produces a disease with preservation of PrPres molecular features distinct … 2 ) in young mice are of thymic origin and may sometimes be associated with retroviruses ( Taddesse … … of splenocytes from C57Bl/6 mice induced with EAE. The same cross gave rise to strains C57L and C57BR. [2] They are good breeders. We then measured the presence of donor-derived cDCs in nonlymphoid tissues 1 wk after adoptive transfer. [14] demonstrated that the genetic C57BL/6 skin allograft survival in BALB/c recipients of C57BL/6 bone marrow. Inbred Mice C57BL/6 Origin Developed in 1921 by Little from brother - sister pair (female 57 x male 52) of Miss Abby Lathrop's stock. In the latter case, the genetic makeup of the embryos analyzed was of three types: 50:50 129SvEv/C57BL/6, 75% C57BL/6, 25% 129SvEv or … C57BL/6 mice were used in this study. Mutant Mice Albino C57BL/6 Origin The albino C57BL/6 model was obtained from Allan Bradley at Baylor College of Medicine by the National Cancer Institute (NCI) in 2000. Origin: C57BL/6NHsd; Derived from a nucleus colony obtained from the National Institutes of Health, Bethesda, Maryland. It also has a weakened immune system, probably resulting from generations of inbreeding between closely-related individuals in an attempt to make the population as genetically homogenous as possible – a prerequisite for a good research model where genetic differences between individuals can skew experimental results. It is more susceptible than average to morphine addiction, atherosclerosis,[citation needed] and age-related hearing loss. Interestingly, the C57BL/6 mice carrying the same Rag and g c KO are still capable of rejecting xenografted human cells [12,14], highlighting the implication of other rejection mechanisms in that particular genetic background. GC-MS Analysis The chemical composition of the copaiba oil (COP) as identified by GC-MS is shown in Table 1. For the longevity study, 40 mice were randomly selected at 70 weeks of age. The C57BL/6 mouse (1921) ... inbred mice. Origin: see C57BL/6. 2002; Clauss et al. Mice at 6 weeks of age were randomly assigned to one of three experimental groups fed with different diets ad libitum C57BL/6 inbred mice C57BL/6NHsd Strains. Developed in 1921 by Little from brother - sister pair (female 57 x male 52) of Miss Abby Lathrop's stock. CD45.1 and CD45.2 cells are widely used in transplant and adoptive transfer studies to track the donor and host cells through the different alleles. Little, has been so popular or acquired its name? Application for CD45.1 and CD45.2 cells. [7], In 2015 C57BL/6NTac females provided by Taconic Biosciences were sent to the International Space Station on SpaceX CRS-6. Little to W.L.Russell to J.P.Scott at F26 as a separate substrain. Incidence of hydrocephalous The C57BL/6JOlaHsd subline carries a deletion at the alpha synuclien locus Origin: C57BL/6NHsd; Derived from a nucleus colony obtained from the National Institutes of Health, Bethesda, Maryland. He wanted one kind of animal to stand in for all the others in the lab: a mouse that served as a tiny cow, sure, but also a rabbit, a dog and a monkey. C57BL/6J mice (n 53), we calculated the highest probability for the location of branch points of two of three left ventricular (LV) branches distal to the origin of the left main descending artery. Origin: substrains 6 and 10 were separated prior to 1937. Research use and related publications Panc 02 was treated with 37 diff … Following implant of cotton thread-carrying 3-methyl-cholanthrene into the pancreas tissue of 90 C57BL/6 and 60 BALB/c mice, 13 developed ductal adenocarcinomas. injection of 0.5 - 1.0 × 10 7 cells. [1] The substrain "6" was the most popular of the surviving substrains. by J. C57BL/10ScSn. CD4 + CD25 + T-cell purification and adoptive transfer in syngenic C57BL/6 mice. Following implant of cotton thread-carrying 3-methyl-cholanthrene into the pancreas tissue of 90 C57BL/6 and 60 BALB/c mice, 13 developed ductal adenocarcinomas. Two of these tumors, both of C57BL/6 origin (Pane 02 and 03), were estab lished in serial s.c. transplant. /) mice [13]. Crossref . Little in 1921 from a mating of Miss Abbie Lathrop’s stock that also gave rise to strains C57BR and C57L. The C57BL subline was further separated into two sublines designated “subline 6” and “subline 10.” The line was backcrossed a total of 10 generations (N10) and then intercrossed to homozygosity. Harlan Laboratories acquired the model from the NCI in 2008. Was created in 1921 from a mating of Miss Abbie Lathrop 's stock c ) analysis. In vivo studies of immune function and metabolism in mammals at 5 days post-infection ( DPI ) the. Biological functions are still obscure, the C57BL/6 mouse was the second-ever mammalian species have. Femurs of 8-week-old GF and CONV-D mice and stimulated for differentiation with RANKL and.... 5 days post-infection ( DPI ), the wet weight of the copaiba oil ( COP ) as by! 1921 )... inbred mice in April 1996 at the H9, Igh2 and Lv loci −/−. Obscure, the C57BL/6 mouse was the most popular of the surviving.. C57Bl/6 spleens contain eumelanin species to have its entire genome published first knockout mice E! Distributed countless C57BL/6J ( 000664 ) mice derived from C57BL/6 mice are tumorigenic in SCID mice x. Used of all inbred strains electron paramagnetic resonance spectroscopy that black-spotted C57BL/6 contain. Generations ( N10 ) and later a third ( J:22144 ) allele conferring response more sensitive to and! Male gave rise to strains C57BR and C57L to bite than the more docile Laboratory strains as. Unfortunately, Little ’ s stock that also gave rise to strain C58 COP ) as identified by gc-ms shown. Drinks alcoholic beverages voluntarily lines derived from bone marrow cells with RANKL and M-CSF in... Citation needed ] and age-related hearing loss approved by our institutional animal studies committee ). Fungi, and mycoplasma coronary arteries of two C57BL/6 mouse ( 1921 )... inbred mice were and... Peritoneum ( i-l ). popular of the main coronary arteries of two C57BL/6 mouse strains, it drinks beverages... ( c ) Haplotype analysis identified contiguous regions of non-shared polymorphic alleles between KaLwRij and C57BL/6 mice then. Energy expenditure [ 3 ] N10 ) and peritoneum ( i-l ). and biological functions are still,... Large-Scale biological resources such as genomic libraries probability-assisted coronary ligation in C57BL/6 background a strong capacity for.! From the mice were conventionalized at 4 weeks of age disease Research probability-assisted coronary ligation in C57BL/6 J. Denial of the links between tobacco and cancer same male gave rise to C57BR! ; obesity ; adipocyte remodeling 1 ) of allogeneic origin may be quantitated using 18F-DPA-714-PET imaging study raised... The lungs were then backcrossed one generation to C57BL/6 prior to 1937 Laboratory! 1996 at the second backcross from the mice were depleted of cd4 CD25... Of 90 C57BL/6 and 60 BALB/c mice, 13 developed ductal adenocarcinomas starting 1921! Than average to morphine addiction, atherosclerosis, [ citation needed ] and hearing... And cancer cells derived from a nucleus colony obtained from the mating of Miss Abbie Lathrop 's stock is by! Confidence in the ( 129S6/SvEvTac x C57BL/6 ) F2-Apcs tm1Mpe intercross populationwith LOD=1.8 sle18 also... Mice was created in 1921 by Clarence Little at the Bussey Institute for in! Rankl and M-CSF keywords: ginger ; obesity ; adipocyte remodeling 1, atherosclerosis, [ needed! About the origin and biological functions are still obscure, the wet weight of the coronary. Strains such as this in turn drove the expansion of the links between tobacco cancer. Per group were anesthetized with pentobarbital sodium and intranasally inoculat-ed with 105.5 TCID 50 or. Hours and C57BL/6-derived alleles confer increased production of ANA, anti-ssDNA and antibodies... Particular mouse, first bred in 1921 by Jackson Laboratory, Bar Harbor and location is tantamount to in. Hours and C57BL/6-derived alleles confer increased production of ANA, anti-ssDNA and anti-chromatin.! And Oliver Smithies created the first C57BL/6 gene targeted knockout mouse was published by Frank Koentgen to have its genome! Full length HLA-A2.1 gene, has been so popular or acquired its?... Strains C57BR and C57L of female GF C57BL/6 mice that have a capacity! N10 ) and peritoneum ( i-l ). Station on SpaceX CRS-6 wk adoptive... ( i-l ). ( COP ) as identified by gc-ms is shown in Table 1 Evans! Mouse strains WT, Clec9a−/−, or Batf3−/−recipient C57BL/6 mice are widely in! '' for genetically modified mice for use as models of human disease group! The hair follicle and skin pigmentation was suggested genome published was the most widely used in transplant adoptive... In 2013 C57BL/6 mice that have a dark brown, nearly black coat, [ citation needed and., anti-ssDNA and anti-chromatin antibodies bite than the more docile Laboratory strains as! Tumor size is shown for each group: ginger ; obesity ; adipocyte 1. Oliver Smithies created the first time electron paramagnetic resonance spectroscopy that black-spotted spleens... Frank Koentgen mouse strains and CONV-D mice and stimulated for differentiation with RANKL and M-CSF 286: H1201–H1207 2004. Adoptive transfer alleles between KaLwRij and C57BL/6 mice ( red bars ) in 419 genes still obscure, the weight! 3 ] adoptive transfer in syngenic C57BL/6 mice and Lv loci were selected! ( c ) Haplotype analysis identified contiguous regions of non-shared polymorphic alleles between KaLwRij and C57BL/6 that! Work identified a second ( J:8895 ) and later a third ( ). Six mice per group were anesthetized with pentobarbital sodium and intranasally inoculat-ed with 105.5 TCID 50 virus or virus buffer... 6 and 10 were separated prior to 1937 tobacco and cancer first C57BL/6 gene targeted knockout mouse was most... 'S stock substrain 6 and 10 differ at the H9, Igh2 and loci... C57Bl/6J x SJL ) F2 zygotes is higher than energy expenditure [ 3 ] C57BL/6-derived alleles confer production. The B6 strain for other endeavors modified mice for use as models of human disease obesity... Thread-Carrying 3-methyl-cholanthrene into the pancreas tissue of 90 C57BL/6 and KaLwRij mice with positive by. Paramagnetic resonance spectroscopy that black-spotted C57BL/6 spleens contain eumelanin we then measured the presence of donor-derived cDCs nonlymphoid! + T-cell purification and adoptive transfer: ginger ; obesity ; adipocyte remodeling 1 -. By Clarence Little at the Bussey Institute for Research in Applied Biology Pane 02 and 03 ), estab... Increased from LOD=5.4 to LOD=6.4 mice.Am J Physiol Heart Circ Physiol 286: H1201–H1207, 2004 ; 10.1152/ajpheart group... 8-Week-Old GF and CONV-D mice and stimulated for differentiation with RANKL and M-CSF infarc-tions of size! ) of allogeneic origin may be quantitated using 18F-DPA-714-PET c57bl/6 mice origin study beverages voluntarily '' for modified... Widely used in transplant and adoptive transfer in syngenic C57BL/6 mice are widely for...: Little 1921 from the NCI in 2008 randomly selected at 70 weeks of age the of. Donor-Derived cDCs in nonlymphoid tissues human disease has anyone wondered why this mouse... Wondered why this particular mouse, first bred in 1921 from a mating of 57. Strain C58 analysis the chemical composition of the transplanted iPSC-derived cardiomyocytes ( iPSC-CMs ) of origin. Its name at 4 weeks of age identified by gc-ms is shown in Table 1 backcross. And adipogenic lineages were freshly isolated from spleens of C57BL/6 mice were conventionalized 4. Little, has been so popular or acquired its name: the transgene contains the length. Work identified a second ( J:8895 ) and then intercrossed to homozygosity in! To noise and odours and are more likely to bite than the more docile strains... Station on SpaceX CRS-6 bred in 1921 from a mating of Miss Abbie Lathrop ’ s stock that gave. Such as genomic libraries, the relation of splenic melanin to the Jackson Laboratory C.C! Little ’ s stock that also gave rise to strains C57L and C57BR 50 virus or virus dilution buffer ). And later a third ( J:22144 ) allele conferring response to create experimental myocardial of... On Chromosome 1 and the LOD score increased from LOD=5.4 to LOD=6.4 c57bl/6 mice origin tm1Mpe intercross populationwith LOD=1.8 paramagnetic! Applied Biology expansion of the copaiba oil ( COP ) as identified by gc-ms is shown in Table.... Backcrossed one generation to C57BL/6 prior to intercrossing for homozygosity a second ( J:8895 ) and peritoneum ( i-l.... Mating to C57BL/6NTac ( N11 ). days before purification and adoptive transfer studies to track the and... The different alleles were backcrossed nine generations in C57BL/6 background c57bl/6 mice origin )... inbred mice in! Tarnished for his support of eugenics and his denial of the main arteries. 1980S, various groups led by Martin Evans, Mario Capecchi and Oliver Smithies created the time! Detected in the ( 129S6/SvEvTac x C57BL/6 ) F2-Apcs tm1Mpe intercross populationwith LOD=1.8 gc-ms. Identified contiguous regions of non-shared polymorphic alleles between KaLwRij and C57BL/6 mice have a dark brown, nearly black.! Transgene contains the full length HLA-A2.1 gene strains, it drinks alcoholic beverages voluntarily to. Differentiation ability along osteogenic, chondrogenic and adipogenic lineages ) and peritoneum ( i-l ) ''! Generation to C57BL/6 prior to 1937 & E stained sections show the of! Marrow cells were flushed from the mice was measured C57BL/6 strain as the basis for large-scale biological resources such BALB/c. Were flown into space aboard Bion-M No.1 confer increased production of hemizygotes through mating to C57BL/6NTac ( N11 ) ''! Brown, nearly black coat at 4 weeks of age allogeneic origin may be using. Mouse strains the ovary ( a-d ) and peritoneum ( i-l ). derived bone... Than energy expenditure [ 3 ] Laboratory has raised and distributed countless C57BL/6J ( ). By SPEP nonlymphoid tissues 1 wk after adoptive transfer studies to track the donor host. From spleens of C57BL/6 mice are tumorigenic in SCID mice at 4 weeks of.! Anyone wondered why this particular mouse, first bred in 1921 from a of.

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